Neonatal diethylstilbestrol treatment alters the estrogen-regulated expression of both cell proliferation and apoptosis-related proto-oncogenes (c-jun, c-fos, c-myc, bax, bcl-2, and bcl-x) in the hamster uterus

dc.contributor.authorZheng, Xinglongen_US
dc.contributor.authorHendry, William J. IIIen_US
dc.date.accessioned2012-01-24T17:48:33Z
dc.date.available2012-01-24T17:48:33Z
dc.date.issued1997-04en_US
dc.descriptionClick on the link below to access the article (may not be free).en_US
dc.description.abstractIn the Syrian hamster, neonatal diethylstilbestrol (DES) treatment and then postpubertal estrogen stimulation induces hyperplasia plus apoptosis (preneoplastic responses) and ultimately neoplasia in the endometrial epithelial cell compartment. As part of a project to investigate the molecular and cellular mechanisms responsible for this phenomenon, expression of several proto-oncogenes (c-jun, c-fos, c-myc, bax, bcl-2 and bcl-x) was compared in estrogen-stimulated uteri from control versus neonatally DES-treated hamsters. According to Northern blot analysis of total uterine RNA, levels of the 3.2-kb c-jun and 2.4-kb c-myc transcripts were not altered by neonatal DES treatment. However, the 1.0 kb bax and 2.7 kb bcl-x transcript levels were significantly increased in the neonatally DES-exposed uteri. According to immunohistochemical analysis of paraformaldehyde-fixed and paraffin-embedded tissue sections, levels of c-Jun, c-Fos, c-Myc, Bax, and Bcl-x proteins were enhanced dramatically in both the luminal and glandular epithelial cells of neonatally DES-exposed uteri. In contrast, the immunostaining signal for Bcl-2 protein was decreased consistently in the epithelial cells of neonatally DES-exposed uteri. In conclusion, neonatal DES treatment induced persistent and epithelial cell-specific imbalances in the estrogen-regulated uterine expression of c-jun, c-fos, c-myc, bax, bcl-2, and bcl-x proto-oncogenes. These imbalances likely play a role in the molecular mechanism by which neonatal DES treatment induces altered estrogen responsiveness including hyperplasia, apoptosis, and ultimately neoplasia in the epithelial compartment of the hamster uterus.en_US
dc.description.sponsorshipNCI NIH HHS/ NICHD NIH HHSen_US
dc.description.versionpeer revieweden_US
dc.identifier9101088en_US
dc.identifierCA 60250/ HD 28074en_US
dc.identifier9100024en_US
dc.identifier.citationCell growth & differentiation : the molecular biology journal of the American Association for Cancer Research. 1997 Apr; 8(4): 425-34.en_US
dc.identifier.issn1044-9523en_US
dc.identifier.urihttp://cgd.aacrjournals.org/cgi/content/abstract/8/4/425
dc.identifier.urihttp://hdl.handle.net/10057/4130
dc.language.isoengen_US
dc.publisherAmerican Association for Cancer Researchen_US
dc.relation.ispartofseriesCell growth & differentiation : the molecular biology journal of the American Association for Cancer Researchen_US
dc.rights.holderCopyright © 1997 by American Association of Cancer Researchen_US
dc.sourceNLMen_US
dc.subjectResearch Support, Non-U.S. Gov'ten_US
dc.subjectResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newborn/metabolismen_US
dc.subject.meshApoptosisen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCell Division/drug effectsen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshDiethylstilbestrol/pharmacologyen_US
dc.subject.meshEstrogens, Non-Steroidal/pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMesocricetusen_US
dc.subject.meshProto-Oncogene Proteins/metabolismen_US
dc.subject.meshProto-Oncogene Proteins c-bcl-2/metabolismen_US
dc.subject.meshProto-Oncogene Proteins c-fos/metabolismen_US
dc.subject.meshProto-Oncogene Proteins c-jun/metabolismen_US
dc.subject.meshProto-Oncogene Proteins c-myc/metabolismen_US
dc.subject.meshUterus/drug effectsen_US
dc.subject.meshbcl-2-Associated X Proteinen_US
dc.subject.meshbcl-X Proteinen_US
dc.subject.meshUterus/metabolismen_US
dc.titleNeonatal diethylstilbestrol treatment alters the estrogen-regulated expression of both cell proliferation and apoptosis-related proto-oncogenes (c-jun, c-fos, c-myc, bax, bcl-2, and bcl-x) in the hamster uterusen_US
dc.typeArticleen_US
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