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Development of microsatellite markers for Buffalograss (Buchloƫ dactyloides; Poaceae), a drought-tolerant turfgrass alternative
Hadle, Jacob J. Konrade, Lauren A. Beasley, Rochelle R. Lance, Stacey L. Jones, Kenneth L. Beck, James B.
Hadle, Jacob J.
Konrade, Lauren A.
Beasley, Rochelle R.
Lance, Stacey L.
Jones, Kenneth L.
Beck, James B.
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2016-08
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Article
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Keywords
Buchloe dactyloides,Great Plains,Illumina sequencing,Native turfgrass alternative,Poaceae,Polyploidy,Simple sequence repeat (SSR) markers
Subjects (LCSH)
Citation
Hadle, Jacob J.; Konrade, Lauren A.; Beasley, Rochelle R.; Lance, Stacey L.; Jones, Kenneth L.; Beck, James B. 2016. Development of microsatellite markers for Buffalograss (Buchloƫ dactyloides; Poaceae), a drought-tolerant turfgrass alternative. Applications in Plant Sciences 4(8):1600033
Abstract
Premise of the study: Buchloe dactyloides (Poaceae) is an important component of Great Plains prairies and a popular droughttolerant turfgrass alternative in North America. This species comprises an autopolyploid series, and microsatellite primers were developed to understand the distribution of genetic variation among cytotypes and across its large geographic range.
Methods and Results: Fifteen microsatellite loci were designed and successfully amplified in six B. dactyloides populations. Within-population genetic diversity was comparatively high, consistent with B. dactyloides' life history. Allelic variation at 13 loci was consistent with the cytotype established in chromosome-counted samples.
Conclusions: This variable, interpretable set of loci allows for the determination of multilocus genotype in B. dactyloides individuals of varying cytotype. Data such as these from a range-wide sample set can provide important insights for germplasm conservation and crop improvement in this ecologically and economically important species.
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Ā© 2016 Hadle et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
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Botanical Society of America
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Applications in Plant Sciences;v.4:no.8
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Open Access
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ISSN
2168-0450