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A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited
Windham, Michael D. Pryer, Kathleen M. Poindexter, Derick B. Li, Fay-Wei Rothfels, Carl J. Beck, James B.
Windham, Michael D.
Pryer, Kathleen M.
Poindexter, Derick B.
Li, Fay-Wei
Rothfels, Carl J.
Beck, James B.
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Issue Date
2020--04-01
Type
Article
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Keywords
Chromosome number,Chromosome squash,Cytology,Meiosis,Polyploidy,Species complex
Subjects (LCSH)
Citation
Windham, M. D., K. M. Pryer, D. B. Poindexter, F.-W. Li, C. J. Rothfels, and J. B. Beck. 2020. A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited. Applications in Plant Sciences 8(4): e11342.
Abstract
Premise. Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources—access to a microscope with 1000× magnification is the most limiting factor.
Methods and Results. Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos.
Conclusions. The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.
Table of Contents
Description
Invited special article for the Special Issue "Conducting Botanical Research with Limited Resources: Low-Cost Methods in the Plant Sciences"
Publisher
John Wiley and Sons Inc
Journal
Applications in Plant Sciences
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ISSN
21680450