Loading...
Differential expression at the proteomic level of stem-cell markers and other gene markers in high-grade serous vs. other subtypes of ovarian cancer.
Mukherjee, Lipilekha
Mukherjee, Lipilekha
Citations
Altmetric:
Files
Loading...
Thesis
Adobe PDF, 8.99 MB
Authors
Other Names
Location
Time Period
Advisors
Original Date
Digitization Date
Issue Date
2017-07
Type
Thesis
Genre
Keywords
Subjects (LCSH)
Electronic dissertations
Citation
Abstract
Ovarian carcinoma is the most lethal neoplasm and the fifth leading cause of death in women due
to gynecological malignancies. It is estimated that there are 225,000 new cases diagnosed and
140,000 deaths occurring annually worldwide. Among the different subtypes, widely
heterogeneous high-grade serous ovarian cancer (HGSOC) poses a great challenge to modern
chemotherapy due to its high recurrence rate and resistance to standard treatments. Both of those
causes are linked to the presence of a small subpopulation of cancer stem cells (CSCs) in the tumor
microenvironment. Recent studies suggest that survival timeline and disease recurrence are linked
to the variable expression of both cancer CSC and other gene biomarkers in cancer cells. Therefore,
using Western blot and immunohistochemistry analyses, we assessed the expression of a host of
CSC and other gene products at the whole-cell level in total protein extracts and sub-cellular levels
in PFA-fixed cells from three HGSOC and five non-HGSOC cell lines. We found that no CSCmarkers
were expressed in monolayer two-dimensional cultures that could differentiate HGSOCs
from non-HGSOCs. In fact, no CSC-biomarker proteins were detected in any of the subtypes of
the cancer-cell lines in our studies. Upon assessing other gene biomarkers at the proteomic level,
we observed prominent differential expression patterns among the HGSOC and non-HGSOC
subtypes and even within a subtype but no definite pattern to distinguish HGSOCs from non-
HGSOCs. In conclusion, determining a differential protein expression profile in subtypes of
ovarian cancer requires much further attention in order to provide earlier detection methods and
personalized treatment options for patients.
Table of Contents
Description
Thesis (M.S.)--Wichita State University, Fairmount College of Liberal Arts and Sciences, Dept. of Biological Sciences
Publisher
Wichita State University
Journal
Book Title
Series
Digital Collection
Finding Aid URL
Use and Reproduction
Copyright 2017 by Lipilekha Mukherjee
All rights reserved