The paper presents the excavations of 2016 season at the Mycenaean site of Kastrouli, Phokis. The site has been looted in the past decades and excavated in a very limited scale by the Archaeological Service. It is located on the top of a rocky hill and delimited by a fortification wall, probably constructed in two phases, the earliest of which could be Mycenaean. A hybrid rock-cut and built chamber tomb, excavated at the westernmost part of the fortified area, contained an undisturbed commingled burial of at least fifteen individuals. The main pottery shape is the stirrup jar, and the small finds include Phi and Psi figurines, steatite beads, and fragments of gold foil. The period of the original use of the tomb falls between the LH IIIA 2 and the LH IIIC Early or Advanced. There is some evidence that the tomb has been reused in the Middle Geometric Period.