Oocyte quality is enhanced by hypoglycosylated FSH through increased cell-to-cell interaction during mouse follicle development
Converse, Aubrey Liu, Zhenghui Patel, Jai C. Shakyawar, Sushil K. Guda, Chittibabu Bousfield, George R. Kumar, T. Rajendra Duncan, Francesca E.
Converse, Aubrey
Liu, Zhenghui
Patel, Jai C.
Shakyawar, Sushil K.
Guda, Chittibabu
Bousfield, George R.
Kumar, T. Rajendra
Duncan, Francesca E.
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Time Period
Advisors
Original Date
Digitization Date
Issue Date
2023-11
Type
Article
Genre
Keywords
FSH glycoform,Ovarian follicle,Oocyte quality,Transzonal projections,Mouse
Subjects (LCSH)
Citation
Converse, A., Liu, Z., Patel, J.C., Shakyawar, S.K., Guda, C., Bousfield, G.R., Kumar, T.R., & Duncan, F.E. (2023). Oocyte quality is enhanced by hypoglycosylated FSH through increased cell-to-cell interaction during mouse follicle development. Development, 150(22). https://doi.org/10.1242/dev.202170
Abstract
Macroheterogeneity in follicle-stimulating hormone (FSH) β-subunit N-glycosylation results in distinct FSH glycoforms. Hypoglycosylated FSH$^{21}$ is the abundant and more bioactive form in pituitaries of females under 35 years of age, whereas fully glycosylated FSH$^{24}$ is less bioactive and increases with age. To investigate whether the shift in FSH glycoform abundance contributes to the age-dependent decline in oocyte quality, the direct effects of FSH glycoforms on folliculogenesis and oocyte quality were determined using an encapsulated in vitro mouse follicle growth system. Long-term culture (10-12 days) with FSH$^{21}$ (10 ng/ml) enhanced follicle growth, estradiol secretion and oocyte quality compared with FSH$^{24}$ (10 ng/ml) treatment. FSH$^{21}$ enhanced establishment of transzonal projections, gap junctions and cell-to-cell communication within 24 h in culture. Transient inhibition of FSH$^{21}$-mediated bidirectional communication abrogated the positive effects of FSH$^{21}$ on follicle growth, estradiol secretion and oocyte quality. Our data indicate that FSH$^{21}$ promotes folliculogenesis and oocyte quality in vitro by increasing cell-to-cell communication early in folliculogenesis, and that the shift in in vivo abundance from FSH$^{21}$ to FSH$^{24}$ with reproductive aging may contribute to the age-dependent decline in oocyte quality.
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Publisher
The Company of Biologists Ltd.
Journal
Book Title
Series
Development
v.150 no. 22
v.150 no. 22
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Archival Collection
PubMed ID
DOI
ISSN
1477-9129 (online)
0950-1991 (print)
0950-1991 (print)