Abnormal myofiber morphology and limb dysfunction in claudication

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Koutakis, Panagiotis
Myers, Sara A.
Cluff, Kim
Ha, Duy M.
Haynatzki, Gleb
McComb, Rodney D.
Uchida, Koji
Miserlis, Dimitrios
Papoutsi, Evlampia
Johanning, Jason M.
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Claudication , Walking distance , Myofiber morphology
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Koutakis, Panagiotis; Myers, Sara A.; Cluff, Kim; Ha, Duy M.; Haynatzki, Gleb; McComb, Rodney D.; Uchida, Koji; Miserlis, Dimitrios; Papoutsi, Evlampia; Johanning, Jason M.; Casale, George P.; Pipinos, Iraklis I. 2015. Abnormal myofiber morphology and limb dysfunction in claudication. Journal of Surgical Research, vol. 196:no. 1, 1 June 2015:pp 172–179

Background: Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeatedischemiainthe lower legmuscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance.

Methods: Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength.

Results: Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance.

Conclusions: Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.

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Elsevier B.V.
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Journal of Surgical Research;v.196:no.1
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