Different influence of Ti, PMMA, UHMWPE, and Co-Cr particles on peripheral blood monocytes during periprosthetic inflammation

No Thumbnail Available
Zhang, Kai
Yang, Shang-You
Yang, Shuye
Bai, Ling
Li, Peng
Liu, Dong
Schurman, John R.
Wooley, Paul H.
Issue Date
Aseptic loosening , Wear debris , Cell trafficking , Monocytes , Inflammation
Research Projects
Organizational Units
Journal Issue
Zhang, Kai; Yang, Shang-You; Yang, Shuye; Bai, Ling; Li, Peng; Liu, Dong; Schurman, John R.; Wooley, Paul H. 2015. Different influence of Ti, PMMA, UHMWPE, and Co-Cr particles on peripheral blood monocytes during periprosthetic inflammation. Journal of Biomedical Materials Research, Part A, vol. 103:no. 1:pp 358–364

This study investigated cellular trafficking and inflammatory markers in orthopedic biomaterial particle-challenged human peripheral blood monocytes (PBMCs) using a murine immunodeficiency (SCID) model. Periprosthetic tissues from aseptic loosening patients were transplanted into muscles of SCID mice. PBMCs from the same patients were stimulated in vitro with Ti-6Al-4V, PMMA, UHMWPE, or Co-Cr particles for 3 days before administered intraperitoneally to the periprosthetic tissue-implanted mice. The xenografts were harvested 2 weeks later for histological and molecular analyses. Significant cell infiltration was obvious in the transplanted tissues from mice transfused with Ti-alloy, PMMA and UHMWPE-provoked PBMCs compared to controls, and UHMWPE-provoked PBMCs group accumulated significantly more cells among all groups. There were ubiquitous TRAP+ stained cells in all xenografts from particle-stimulated PBMCs mice. Immunohistochemical staining indicated that significantly more IL-1 and TNF positive cells occurred in Ti and PMMA groups; while the UHMWPE group resulted in stronger positive MCP-1 and IL-6 stains. Polymerase chain reaction (PCR) confirmed overexpression of both IL-1 and TNF in Ti and PMMA-stimulated groups; and more MIP-1 gene expression developed in the UHMWPE group. Overall, different type of orthopedic materials influenced the trafficking ability of particle-activated PBMCs which may depend on upregulation of various proinflammatory cytokines and chemokines.

Table of Contents
Click on the DOI link to access the article (may not be free).
Wiley Periodicals, Inc.
Book Title
Journal of Biomedical Materials Research;v.103:no.1
PubMed ID