CE-LIF coupled with PDMS-interconnected microfluidic systems for rapid separations of neurotransmitters

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Zhang, Qiyang
Maddukuri, Naveen
Gong, Maojun

Zhang, Q., & Maddukuri, N. CE-LIF Coupled with PDMS-interconnected Microfluidic System for Rapid Separations of Neurotransmitters. -- In Proceedings: 10th Annual Symposium on Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p. 172


In vivo measurement of neurotransmitters in cerebrospinal fluidic (CSF) is an effective method to monitor neuronal activities. Microdialysis coupled with HPLC or capillary electrophoresis (CE) is a powerful approach for in vivo monitoring of neurotransmitters. HPLC requires relative large sample volumes (e.g. 5-15 ?L), which is infeasible for limited availability of CSF [1], whereas CE is able to analyze nano- or pico-liter samples in a short time such as 20 seconds. We have developed an integrated CE system targeting on in vivo measurements of essential neurotransmitters. This CE system employs laser-induced fluorescence detectors, and online fluorogenic derivatization. Experimental results show that this system is capable of performing long-term monitoring with robustness, accuracy, high sensitivity and reproducibility in a real-time fashion.

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Presented to the 10th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Heskett Center, Wichita State University, April 25, 2014.
Research completed at Department of Chemistry, College of Liberal Arts and Sciences