Prediction of selectivity for the separation of double-stranded DNA fragments in electrophoresis

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Issue Date
1997-09-01
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Authors
Otim, Ochan
Singhal, Ram P.
Advisor
Citation

Journal of capillary electrophoresis. 1997 Sep-Oct; 4(5): 247-51.

Abstract

The electrophoretic resolution (selectivity) of double-stranded DNA (dsDNA) restriction fragments is studied for different organisms (phi X174, lambda phage, and pGEM-3 plasmids) by various separation and detection methods. The selectivity can be predicted by plotting the difference in chain length over their average chain length (base pair difference/average base pair magnitude of delta bp/Ave.-bp) against each DNA fragment pair. Experimental conditions and the nature of the DNA can influence the predicted results.

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