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Transport of tryptophan into SH-SY5Y neuronal cells
Kollalpitiya, Konara Yamuna Wimalasena, Kandatege
Kollalpitiya, Konara Yamuna
Wimalasena, Kandatege
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2008-04-25
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Conference paper
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Kollalpitiya,Yamuna, Wimalasena, Kandatege(2008). Transport of tryptophan into SH-SY5Y neuronal cells. In Proceedings: 4th Annual Symposium: Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p.149-150
Abstract
The human neuroblastoma cell line SH-SY5Y is a commonly used catecholaminergic model in studies related to
neurotoxicity of various substances, oxidative stress, and neurodegenerative diseases. Specific transport of monoamine
neurotransmitter precursors i.e. corresponding aromatic amino acids, into these cells is essential for cellular metabolism.
Serotonin (5-hydroxytryptamine) is a neurotransmitter synthesized in the brain that is implicated in many brain disorders
including Schizophrenia and manic depression. Serotonin is synthesized from tryptophan in serotonergic neurons. In the
present study we have examined the uptake of L-tryptophan into SH-SY5Y cells by reverse phase high performance liquid
chromatography coupled with UV detection. This study shows that SH-SY5Y cells possess significant capacity to take up
extracellular tryptophan. The tryptophan uptake was inhibited by a specific inhibitor of system L, a transport system present
in mammalian cells serves for neutral amino acids, providing evidence that tryptophan enters the cells via transport system
L. Both undifferentiated and 12-O-tetradecanoly-phorbol-12-acetate (TPA) differentiated SH-SY5Y cells show
similar tryptophan uptake efficiencies.
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Description
Paper presented to the 4th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Hughes Metropolitan Complex, Wichita State University, April 25, 2008.
Research completed at the Department of Chemistry, Fairmount College of Liberal Arts and Sciences
Research completed at the Department of Chemistry, Fairmount College of Liberal Arts and Sciences
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Wichita State University. Graduate School.
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GRASP
v.4
v.4