Effects of hypoxia on proliferation and apoptosis of osteosarcoma cells
Yang, Shang-You Garcia, Elka S. Xia, Will Wang, Anyu
Yang, Shang-You
Garcia, Elka S.
Xia, Will
Wang, Anyu
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Time Period
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Original Date
Digitization Date
Issue Date
2021-09-30
Type
Article
Genre
Keywords
Hypoxia,Osteosarcoma,HIF-1α,VEGF,Apoptosis
Subjects (LCSH)
Citation
Yang, S. -., Garcia, E., Xia, W., & Wang, A. (2021). Effects of hypoxia on proliferation and apoptosis of osteosarcoma cells. Anticancer Research, 41(10), 4781-4787. doi:10.21873/anticanres.15293
Abstract
Background: Hypoxia can happen during solid tumor growth including osteosarcoma. This study investigated the relationship of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) on osteosarcoma cell growth and apoptosis under hypoxic conditions. Materials and Methods: Human osteosarcoma cells were cultured under normal or hypoxic conditions. Inhibitors of HIF-1α and VEGF were applied to the cells separately or in combination to block the respective proteins. Cell proliferation and apoptosis were examined by MTT and TUNEL assays, and real-time PCR and ELISA were performed for mRNA and protein expression. Results: There was a dramatic decrease of cell proliferation and an elevation of apoptosis under hypoxia. Blockage of HIF-1α and VEGFR enhanced the cell growth retardation and promoted apoptotic changes. Moreover, blockage of HIF-1α significantly eliminated the expression of VEGF in the cell culture media, and vice versa. Conclusion: HIF-1α and VEGF work closely in regulating osteosarcoma cell growth under hypoxic conditions and blockage of either of them may subsequently influence the presence of the other.
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Publisher
International Institute of Anticancer Research
Journal
Book Title
Series
Anticancer Research;Vol. 41, Iss. 10
Digital Collection
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Archival Collection
PubMed ID
DOI
ISSN
0250-7005
1791-7530
1791-7530