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The effect of glioma extracellular matrix components on tumor cell migration
Neupane, Nischal
Neupane, Nischal
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2025-05
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Aggressive primary brain tumors starting from glial cells in the central nervous system are called gliomas. With collagen and hyaluronic acid as main components, the extracellular matrix (ECM) is vital in controlling tumor cell behavior. While hyaluronic acid offers a hydrated, elastic environment and signals by CD44 and RHAMM receptors to promote cytoskeletal reorganization, proliferation, and invasion, collagen acts as a structural scaffold and influences signaling pathways via integrin-mediated interactions. On collagen, hyaluronic acid, and collagen-hyaluronic acid composite substrates, this work examined the migratory and proliferative responses of U87 glioma cells and primary glioma cells. Results revealed that glioma cells show higher migration speed and proliferation rate on collagen-coated surfaces compared with hyaluronan coated surface. Combining hyaluronic acid with collagen produced an intermediary phenotype. High doses of ascorbic acid were investigated in relation to glioma cell behavior. As an antioxidant, ascorbic acid worked at low dosages to lower reactive oxygen species (ROS) levels, so promoting cell survival. High concentrations, however, had a prooxidant effect that caused cytotoxicity, interfered with mitochondrial function, and resulted in death. These results have important consequences for the design of anti-glioma treatments since they imply that aiming at ECM-cell interactions or changing ECM stiffness and composition could lower tumor invasiveness.
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Thesis (M.S.)-- Wichita State University, College of Liberal Arts and Sciences, Dept. of Biological Sciences
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Wichita State University
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