Exploration of molecular pathways mediating electric field-directed Schwann cell migration by RNA-Seq

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Authors
Yao, Li
Li, Yongchao
Knapp, Jennifer R.
Smith, Peter
Advisors
Issue Date
2015-07
Type
Article
Keywords
Spinal-cord-injury , Activated protein-kinase , Expression analysis , Epithelial-cells , Motility , Myelination , Iqgap1 , Keratinocytes , Chemotaxis , Receptors
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Citation
Yao, L., Li, Y., Knapp, J. and Smith, P. (2015), Exploration of molecular pathways mediating electric field-directed schwann cell migration by RNA-seq. J. Cell. Physiol., 230: 1515–1524. doi: 10.1002/jcp.24897
Abstract

In peripheral nervous systems, Schwann cells wrap around axons of motor and sensory neurons to form the myelin sheath. Following spinal cord injury, Schwann cells regenerate and migrate to the lesion and are involved in the spinal cord regeneration process. Transplantation of Schwann cells into injured neural tissue results in enhanced spinal axonal regeneration. Effective directional migration of Schwann cells is critical in the neural regeneration process. In this study, we report that Schwann cells migrate anodally in an applied electric field (EF). The directedness and displacement of anodal migration increased significantly when the strength of the EF increased from 50mV/mm to 200mV/mm. The EF did not significantly affect the cell migration speed. To explore the genes and signaling pathways that regulate cell migration in EFs, we performed a comparative analysis of differential gene expression between cells stimulated with an EF (100mV/mm) and those without using next-generation RNA sequencing, verified by RT-qPCR. Based on the cut-off criteria (FC>1.2, q<0.05), we identified 1,045 up-regulated and 1,636 down-regulated genes in control cells versus EF-stimulated cells. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis found that compared to the control group, 21 pathways are down-regulated, while 10 pathways are up-regulated. Differentially expressed genes participate in multiple cellular signaling pathways involved in the regulation of cell migration, including pathways of regulation of actin cytoskeleton, focal adhesion, and PI3K-Akt. J. Cell. Physiol. 230: 1515-1524, 2015.

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Publisher
John Wiley & Sons, Inc.
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Series
Journal of Cellular Physiology;v.230:no.7
PubMed ID
DOI
ISSN
0021-9541
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