Isolation and Characterization of Coproporphyrin Produced by Four Subspecies of Bacillus thuringiensis

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Issue Date
1986-03
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Authors
Harms, R. L.
Martinez, D. R.
Griego, V. M.
Advisor
Citation

Applied and environmental microbiology. 1986 Mar; 51(3): 481-6.

Abstract

It was found by using spectrophotometric, spectrofluorometric, and high-pressure liquid chromatography that four subspecies of Bacillus thuringiensis produce coproporphyrin. The porphyrin isomer was identified as coproporphyrin I for B. thuringiensis subsp. kurstaki (HD1). The porphyrin was isolated both from spores and from a variety of spent growth media. The quantity of porphyrin released by each Bacillus subspecies differed. The rank order of porphyrin production follows: B. thuringiensis subsp. kurstaki HD1 > B. thuringiensis subsp. thuringiensis HD27 > B. thuringiensis subsp. thuringiensis HD41 > B. thuringiensis subsp. darmstadiensis HD199.

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