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Rheological characterization and print quality analysis studies of Gelatin/collagen I/PEGDA hydrogels for bioprinting tumor models for cancer therapy development
Bartholomew, Kami
Bartholomew, Kami
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t24003_Bartholomew.pdf
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2024-05
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Additive manufacturing, also known as 3D printing, is a technology that is used to create rapid prototypes and customized parts. This technology has recently been used in the field of tissue engineering and is called bioprinting. Bioprinting is an improvement on older tissue engineering techniques as it allows cells to be evenly distributed throughout the entire construct and has been used to create models of diseased tissue, test novel drugs, create vasculature, and create potentially transplantable tissues and organs. Interest in bioprinting has grown in a variety of medical research areas including cancer drug development. In the past 2D cell culture has been used to study cancerous cells and test their responses to cancer therapies before moving to animal and human trials. Over the years it has been found that results obtained from 2D models are not consistent with the results obtained from in vivo trials. The solution is to create 3D models that better recapitulate the in vivo condition. This can be done using bioprinted models as their shape, size, cell type, and material composition can be customized. The materials used for bioprinting are called bioinks. While there have been improvements in bioink formulations, there is still a need to develop bioinks to be used in a variety of applications. Therefore, the objective of this thesis is to develop a unique bioink formulation and investigate its properties and analyze the quality of the bioprinted constructs created using each bioink formulation. The formulations developed contained gelatin, collagen I, and different concentrations of PEGDA. The results showed that all formulations had acceptable rheological properties, with the addition of PEGDA causing higher degrees of shear-thinning, better viscosity recovery, and decreased viscosity. After conducting print quality analysis, it was determined that formulation P5 has the best overall print quality, making it the most suitable for creating bioprinted constructs.
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Thesis (M.S.)-- Wichita State University, College of Engineering, Dept. of Biomedical Engineering
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Wichita State University
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