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Genus-wide microsatellite primers for the Goldenrods (Solidago; Asteraceae)
Beck, James B. Semple, John C. Brull, Justin M. Lance, Stacey L. Phillips, Mai M. Hoot, Sara B. Meyer, Gretchen A.
Beck, James B.
Semple, John C.
Brull, Justin M.
Lance, Stacey L.
Phillips, Mai M.
Hoot, Sara B.
Meyer, Gretchen A.
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2014-04
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Article
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Keywords
Asteraceae,Illumina sequencing,Polyploidy,Simple sequence repeat (SSR) markers,Solidago
Subjects (LCSH)
Citation
Abstract
Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation
genetics, and species delimitation in the genus Solidago .
• Methods and Results: Illumina sequencing of a shotgun library from S. gigantea identifi ed ca. 1900 putative single-copy loci.
Fourteen loci were subsequently shown to be amplifi able, single-copy, and variable in a broad range of Solidago species.
• Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate
numerous inter- and intraspecifi c studies in the ca. 120 Solidago species.
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© 2014 Beck et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
Publisher
Botanical Society of America
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Applications in Plant Sciences;v.2 no.4
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2168-0450