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Effects of follicle-stimulating hormone glycosylation on ovarian follicle development
Cosper, Kirsten Elisabeth
Cosper, Kirsten Elisabeth
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2023-12
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Follicle-stimulating hormone (FSH) is a reproductive glycoprotein hormone that exhibits macroheterogeneity in the form of absence or presence of a glycan. Three glycoforms of FSH exist, including FSH18, FSH21, and FSH24, with FSH24 being the least bioactive form of FSH and FSH21 being the most bioactive form. This is physiologically significant, because FSH21 is the dominant circulating form of FSH in young women of reproductive maturity, however, as women approach perimenopause, FSH24 becomes the dominant circulating form.
FSH exhibits stage specific functions in regard to ovarian follicle development. This study will measure the expression of two genes, connexin-43 (GJA1), and hyaluron synthase 2 (HAS2). Connexin-43 is upregulated during early stages of follicle development to induce gap junction communication and downregulated during late stages of follicle development to halt gap junction communciation. Hyaluron synthase 2 (HAS2) is upregulated during late stages of follicle development to induce cumulus expansion, a morphological change in the cumulus-oocuyte complex that prepares the oocyte for ovulation. Porcine ovaries were used to isolate cumulus-oocyte complexes and granulosa cells to be used for quantitative real time PCR studies. The cumulus- oocyte complexes and granulosa cells were treated with FSH18, FSH21, and FSH24 and the expression of gap junction protein connexin-43 and cumulus expansion-associated protein hyaluronan synthase-2 were measured and compared to investigate how glycosylation of FSH effects the expression of these vital genes involved in ovarian follicle development.
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Thesis (M.S.)-- Wichita State University, College of Liberal Arts and Sciences, Dept. of Biological Sciences
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Wichita State University
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© Copyright 2023 by Kirsten Elisabeth Cosper
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