Influence of size on apparent scrambling of sequence during CID of b-type ions
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Authors
Molesworth, Samuel P.
Osburn, Sandra M.
Van Stipdonk, Michael J.
Advisors
Issue Date
2009-11-01
Type
Article
Keywords
Research Support, Non-U.S. Gov't , Research Support, U.S. Gov't, Non-P.H.S.
Citation
Journal of the American Society for Mass Spectrometry. 2009 Nov; 20(11): 2174-81.
Abstract
We investigated the influence of peptide size on the apparent loss of sequence during collision-induced dissociation (CID) of b ions using a group of peptides containing from between 4 and 10 residues. Although scrambling of sequence for b3+ generated from tetrapeptides is minimal, significant formation of nondirect sequence ions (i.e., ions for which scrambling has apparently occurred) was observed for all larger b ions included in the study.
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Publisher
Springer
Journal
Book Title
Series
Journal of the American Society for Mass Spectrometry
J. Am. Soc. Mass Spectrom.
J. Am. Soc. Mass Spectrom.
PubMed ID
DOI
ISSN
1879-1123
1044-0305
1044-0305