To understand the disease mechanisms of charcoal rot caused by Macrophomina phaseolina at the molecular level, we established a pathosystem using the model plant Arabidopsis thaliana. The cellular infection process and pathogen propagation within the host system during the early and late stages of infection were examined by microscopy. Studies have shown that phytohormones play crucial role in the induction of defense signaling pathways. In this study, we use a genetics approach to understand the mechanisms of plant immunity against Macrophomina mediated by plant hormones.