Introduction: This study is designed to determine if a relationship exists between increased standing during class lecture time and gluteus medius strength, abdominal strength, hip flexor flexibility, hamstring flexibility, gastrocnemius flexibility, back pain, or physical activity level in healthy students aged 18-35 years. Methods: Twenty participants were recruited from the WSU Doctor of Physical Therapy program. Ten subjects used standing desks for 75% of class lecture time for 12 weeks. They were matched to 10 control subjects who sat throughout class lectures. Dynamometry, goniometry, inclinometry, and questionnaires were used for objective measurements assessed at weeks 0, 6, and 12. Results/Conclusion: Standing during 75% of class lecture time induced statistically significant increases in hip abductor strength, decreases in back pain, and a clinically significant increase in hamstring flexibility over 12 weeks compared to controls. No detrimental changes were found in the seven studied variables with a 3:1 standing-to-sitting ratio.