Affinity chromatography of glucose dehydrogenase

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Issue Date
1988-01-15
Embargo End Date
Authors
Carper, W. Robert
Groutas, William C.
Coffin, D.B.
Advisor
Citation

Experientia. 1988 Jan 15; 44(1): 29-32.

Abstract

Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies.

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