Endothelial cells (ECs) are heterogeneous structures lining the blood vessels of the vasculature exposed to a complex microenvironment of forces due to blood flow such as fluid shear stress (FSS) which impacts cell morphology, mechanics, physiology, and pathology. Gaining insight into the forces cells are exposed to and the process of mechanotransduction is key to better understanding these impacts. Therefore, the objective of this research was to develop a one-way fluid structure interaction (FSI) co-simulation framework that can predict the FSS distribution on the EC surface and the resulting displacement and von Mises stress distributions in an idealized multicomponent EC finite element analysis (FEA). A computational fluid dynamics (CFD) FEA of a parallel plate flow chamber was validated for steady, unidirectional, and laminar flow resulting in FSS values of 0.3, 16, and 32 dyn/cm2. Additionally, an idealized multicomponent (cell membrane, nucleus, cytoplasm, focal adhesions) EC structural FEA with viscoelastic material properties was verified for predicting displacement and von Mises stress distribution under shear load. These two FEA models were then used to conduct a one-way FSI co-simulation. The FSS distribution on the EC was found to be heterogeneous, with max FSS occurring at the peak height of the EC and increasing as the inlet flow rate increased. Furthermore, as the FSS increased, displacement and von Mises stress distributions in the EC also increased with max displacements corresponding to the location of max FSS. Additionally, stress amplifications were observed at focal adhesions and the nucleus-cytoplasm interface. The results presented show the effectiveness of the developed co-simulation framework to expose an idealized EC FEA to physiological microvasculature FSS values. The co-simulation framework can be expanded to predict FSS for different flow conditions and the resultant deformation and von Mises stress distribution of an EC with cell-specific geometry, other subcellular structures, or other types of adherent cells.