This study aimed to synthesize and manufacture electrically conductive electrospun fibers for wearable health monitoring devices. Wearable health monitoring devices are essential for early and quick detection of diseases. High electrical conductivity is crucial for their performance and fast response. Electrically conductive nanofibers have the potential to enhance the performance of wearable biosensors. In this study, 18 different nanofibers were synthesized by electrospinning. PAN, SPEEK, PVDF, and polystyrene fibers were prepared, and different weight ratios of PEG and PVP inclusions were added with PVDF and PS to make fibers hydrophilic. It was observed that both PVDF and PS fibers showed hydrophilic characteristics after adding hydrophilic polymers into the solution at a certain amount. The measured water contact angle (WCA) for many fibers was 0°. The synthesized fibers were then dip-coated in an electrically conductive PEDOT: PSS solution under different conditions. Ultrasonication and desiccation effects were applied to enhance the impact of the dip coating procedure. It was found that ultrasonication and desiccation effect during the dip coating process has an impact on adsorbing the conductive solution and enhancing the electrical conductivity of the fibers. In this study, PAN fiber showed the highest electrical conductivity of 23.08 S/cm among all fibers. Polystyrene and PVDF-based fibers also showed good electrical conductivity of 17.52 S/cm and 6.72 S/cm, respectively. In this work, four-point probe test, WCA, FTIR, SEM, and EDS tests were performed to study fiber morphology. The conductive fibers prepared in this study have promising applications in wearable health monitoring devices and biosensors applications. This study may open novel opportunities for preparing electrically conductive fibers and their applications.