Investigation of intramolecular proton migration in a series of model, metal-cationized tripeptides using in situ generation of an isotope label

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Authors
Bulleigh, Kellis
Howard, Angela
Do, Trang
Wu, Qun
Anbalagan, Victor
Van Stipdonk, Michael J.
Advisors
Issue Date
2006-01-01
Type
Article
Keywords
Research Support, Non-U.S. Gov't , Research Support, U.S. Gov't, Non-P.H.S.
Research Projects
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Citation
Rapid communications in mass spectrometry : RCM. 2006; 20(2): 227-32.
Abstract

In this study we used an isotope label, generated in situ, to investigate intramolecular proton migration or scrambling during formation of b(2)+17+Li products by collision-induced dissociation (CID) of Li(+)-cationized tripeptides. To generate the isotope label, we used a McLafferty-type rearrangement of N-terminally acetylated, C-terminal peptide tert-butyl esters in which all amide positions were exchanged with deuterium. Using a set of small, model peptides, we show that intramolecular proton scrambling occurs during CID, particularly amongst adjacent sites along a peptide backbone, on the time scales employed for low-energy collisional activation in an ion-trap mass spectrometer.

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Publisher
John Wiley and Sons
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Book Title
Series
Rapid communications in mass spectrometry : RCM
Rapid Commun. Mass Spectrom.
PubMed ID
DOI
ISSN
0951-4198
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