An electrospun polyaniline nanofiber as a novel platform for real-time COX-2 biomarker detection
Veisi, Zeinab Ceylan, Muhammet Mahapatro, Anil Asmatulu, Ramazan
Veisi, Zeinab
Ceylan, Muhammet
Mahapatro, Anil
Asmatulu, Ramazan
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Time Period
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Original Date
Digitization Date
Issue Date
2014
Type
Conference paper
Genre
Keywords
Cancer,Cyclooxygenase-2,Cells,Biosensor,Pathway,Target,Key
Subjects (LCSH)
Citation
Veisi, Zeinab; Ceylan, Muhammet; Mahapatro, Anil; Asmatulu, Ramazan. 2014. An electrospun polyaniline nanofiber as a novel platform for real-time COX-2 biomarker detection. Proceedings of the ASME 2013 International Mechanical Engineering Congress and Exhibition (IMECE2013), vol. 3B
Abstract
The presence of Cyclooxygenase-2 (COX-2) biomarker has been associated with the development of certain types of cancer such as breast cancer. Moreover, reliable quantification of COX-2 as an enzyme responsible for pain and inflammation is vital. Here we demonstrate the feasibility of sensitive COX-2 detection via integration of nanoporous polyaniline fibers on the microfabricated platform to develop a label-free biosensor. Highly porous polyaniline nanofibers were fabricated in different diameters and integrated on the interdigitated microelectrodes to develop electrochemical platforms. Characterization results revealed that the smaller diameter improved the sensitivity of the biosensor due to enhancement in the specific surface area. The developed biosensor was able to detect analyte as low as 0.1 pg/mL with a large dynamic linear range of 10 fg/mL to 1 mu g/mL. The fabricated sensor showed remarkable sensitivity towards COX-2 antigen suggesting the significant contribution of this nanofiber based platform to the enhanced sensitivity in COX-2 analyte detection.
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Publisher
American Society of Mechanical Engineers
Journal
Book Title
Series
Proceedings of the ASME 2013 International Mechanical Engineering Congress and Exhibition (IMECE2013);v.3B