Interpretation of gram-stained sputa containing Moraxella (Branhamella) catarrhalis
Ainsworth, Samuel M. Nagy, Stephanie B. Morgan, Loretta A. Miller, Glendon R. Perry, Jack L.
Ainsworth, Samuel M.
Nagy, Stephanie B.
Morgan, Loretta A.
Miller, Glendon R.
Perry, Jack L.
Other Names
Location
Time Period
Advisors
Original Date
Digitization Date
Issue Date
1990-11
Type
Article
Genre
Keywords
Subjects (LCSH)
Citation
Journal of clinical microbiology. 1990 Nov; 28(11): 2559-60.
Abstract
Sputum specimens culture positive for Moraxella (Branhamella) catarrhalis were Gram stained with three decolorizer solutions (slow, 95% ethyl alcohol; intermediate, 1:1 ratio of 95% ethyl alcohol and acetone; and fast, acetone alone) for 5, 10, 20, and 30 s. Optimal results were obtained with acetone alone after 10 s or with a 1:1 mixture of acetone and ethanol after 20 s. Inadequate decolorization of M. catarrhalis in sputa is likely if the decolorization solution and exposure time are not optimal and may contribute to underreporting of this organism.
Table of Contents
Description
Click on the link below to access the article (may not be free).
Publisher
American Society for Microbiology
Journal
Book Title
Series
Journal of clinical microbiology
Digital Collection
Finding Aid URL
Use and Reproduction
Archival Collection
NLM
PubMed ID
DOI
ISSN
0095-1137