Interpretation of gram-stained sputa containing Moraxella (Branhamella) catarrhalis
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Authors
Ainsworth, Samuel M.
Nagy, Stephanie B.
Morgan, Loretta A.
Miller, Glendon R.
Perry, Jack L.
Advisors
Issue Date
1990-11
Type
Article
Keywords
Citation
Journal of clinical microbiology. 1990 Nov; 28(11): 2559-60.
Abstract
Sputum specimens culture positive for Moraxella (Branhamella) catarrhalis were Gram stained with three decolorizer solutions (slow, 95% ethyl alcohol; intermediate, 1:1 ratio of 95% ethyl alcohol and acetone; and fast, acetone alone) for 5, 10, 20, and 30 s. Optimal results were obtained with acetone alone after 10 s or with a 1:1 mixture of acetone and ethanol after 20 s. Inadequate decolorization of M. catarrhalis in sputa is likely if the decolorization solution and exposure time are not optimal and may contribute to underreporting of this organism.
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Description
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Publisher
American Society for Microbiology
Journal
Book Title
Series
Journal of clinical microbiology
PubMed ID
DOI
ISSN
0095-1137