Exercise induced hypoalgesia (EIH) is the observed effect of lowered pain sensitivity following high-intensity or long-duration exercise. Blood flow restriction (BFR) is the procedure of using inflatable cuffs to partially reduce blood flow to the extremities. When BFR is combined with low-intensity exercise, it mimics the effects of high-intensity exercise; this makes it easier for populations that cannot tolerate high-intensity exercise, such as recovering patients, to relieve their pain. Recent studies have shown that the effects of BFR exercise can last up to one hour post-exercise. However, little evidence has been gathered studying the duration and magnitude of EIH due to BFR exercise across a 24-hour period. Therefore, this study aims to assess pain sensitivity in a 24-hour period following exercise to gain an understanding of the EIH response following BFR exercise. The change from pre-exercise values for pain pressure tolerance of the biceps brachii and quadriceps muscle group was calculated 4-, 8-, and 24-hours post-exercise. Within the control intervention, where patients remained seated with no exercise, the average change in quadricep pain pressure tolerance was 0.25 kg of force (Δkgf) at 4-hours, 1.14 Δkgf at 8-hours, and 1.26 Δkgf at 24-hours. The average change in biceps brachii pain pressure tolerance during the control intervention was 0.37 Δkgf at 4-hours, 0.68 Δkgf at 8-hours, and 0.52 Δkgf at 24-hours. Within the BFR intervention, where patients completed leg extensions at 30% maximal strength with the cuffs inflated to 60% maximum occlusion pressure, the average change in quadricep pain pressure tolerance was –0.07 Δkgf at 4-hours, 0.34 Δkgf at 8-hours, and 1.28 Δkgf at 24-hours. The average change in biceps brachii pain pressure tolerance during the BFR intervention was 0.12 Δkgf at 4-hours, 0.11 Δkgf at 8-hours, and 1.07 Δkgf at 24-hours. While a small sample size (n=4) means inconclusive data, more data is being collected and analyzed. With larger numbers, this study could help develop more accessible pain management in rehabilitation settings.