Rapid labeling of amino acid neurotransmitters with a fluorescent thiol in the presence of o-phthalaldehyde
Maddukuri, Naveen Zhang, Qiyang Zhang, Ning Gong, Maojun
Maddukuri, Naveen
Zhang, Qiyang
Zhang, Ning
Gong, Maojun
Citations
Altmetric:
Other Names
Location
Time Period
Advisors
Original Date
Digitization Date
Issue Date
2017-02
Type
Article
Genre
Keywords
Flow-gate capillary electrophoresis,Fluorescent labeling,Laser-induced fluorescence,Microdialysis,Neurotransmitter
Subjects (LCSH)
Citation
Maddukuri, N., Zhang, Q., Zhang, N. and Gong, M. (2017), Rapid labeling of amino acid neurotransmitters with a fluorescent thiol in the presence of o-phthalaldehyde. ELECTROPHORESIS, 38: 507–512
Abstract
LIF detection often requires labeling of analytes with fluorophores; and fast fluorescent derivatization is valuable for high-throughput analysis with flow-gated CE. Here, we report a fast fluorescein-labeling scheme for amino acid neurotransmitters, which were then rapidly separated and detected in flow-gated CE. This scheme was based on the reaction between primary amines and o-phthalaldehyde in the presence of a fluorescent thiol, 2-((5-fluoresceinyl) aminocarbonyl) ethyl mercaptan (FACE-SH). The short reaction time (<30 s) was suited for on-line mixing and derivatization that was directly coupled with flow-gated CE for rapid electrophoretic separation and sensitive LIF detection. To maintain the effective concentration of reactive FACE-SH, Tris(2- carboxyethyl) phosphine was added to the derivatization reagents to prevent thiol loss due to oxidation. This labeling scheme was applied to the detection of neurotransmitters by coupling in vitro microdialysis with online derivatization and flow-gated CE. It is also anticipated that this fluorophore tagging scheme would be valuable for on-chip labeling of proteins retained on support in SPE.
Table of Contents
Description
Click on the DOI link to access the article (may not be free).
Publisher
John Wiley & Sons, Inc.
Journal
Book Title
Series
Electrophoresis;v.38:no.3-4
Digital Collection
Finding Aid URL
Use and Reproduction
Archival Collection
PubMed ID
DOI
ISSN
0173-0835