Alternative method for isolation of double-stranded template for DNA sequencing
Sadiev, Sagypash Taylor, Alan
Sadiev, Sagypash
Taylor, Alan
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Original Date
Digitization Date
Issue Date
1996-08
Type
Article
Genre
Keywords
Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.
Subjects (LCSH)
Citation
BioTechniques. 1996 Aug; 21(2): 233-5.
Abstract
The increased use of plasmid DNAs
in recombinant DNA technology, coupled
with the growing popularity of
double-stranded DNA sequencing, necessitates
the development of rapid, inexpensive
and efficient procedures for
the isolation of highly purified plasmids.
Numerous miniprep procedures
have been described for preparing plasmid
DNAs (1,2,4,5,8,9). A quick and
efficient alternative method for the
preparation of large quantities of purified
plasmid DNA is presented in this
report.
Plasmid isolated by this
method is of sufficient quality for use
in DNA sequencing reactions and other
molecular biological techniques. This
procedure provides an attractive alternative
to more expensive and/or timeconsuming
methods currently used to
prepare plasmid DNA.
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Publisher
Informa Healthcare
Journal
Book Title
Series
BioTechniques
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NLM
PubMed ID
DOI
ISSN
0736-6205