Alternative method for isolation of double-stranded template for DNA sequencing

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Authors
Sadiev, Sagypash
Taylor, Alan
Advisors
Issue Date
1996-08
Type
Article
Keywords
Research Support, Non-U.S. Gov't , Research Support, U.S. Gov't, Non-P.H.S.
Research Projects
Organizational Units
Journal Issue
Citation
BioTechniques. 1996 Aug; 21(2): 233-5.
Abstract

The increased use of plasmid DNAs in recombinant DNA technology, coupled with the growing popularity of double-stranded DNA sequencing, necessitates the development of rapid, inexpensive and efficient procedures for the isolation of highly purified plasmids. Numerous miniprep procedures have been described for preparing plasmid DNAs (1,2,4,5,8,9). A quick and efficient alternative method for the preparation of large quantities of purified plasmid DNA is presented in this report. Plasmid isolated by this method is of sufficient quality for use in DNA sequencing reactions and other molecular biological techniques. This procedure provides an attractive alternative to more expensive and/or timeconsuming methods currently used to prepare plasmid DNA.

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Publisher
Informa Healthcare
Journal
Book Title
Series
BioTechniques
PubMed ID
DOI
ISSN
0736-6205
EISSN