Differential ion mobility separations/mass spectrometry with high resolution in both dimensions

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Authors
Baird, Matthew A.
Anderson, Gordon A.
Shliaha, Pavel V.
Jensen, Ole N.
Shvartsburg, Alexandre A.
Advisors
Issue Date
2019-01-15
Type
Article
Keywords
Dissociation , Mass spectrometry
Research Projects
Organizational Units
Journal Issue
Citation
Baird, Matthew A.; Anderson, Gordon A.; Shliaha, Pavel V.; Jensen, Ole Noerregaard; Shvartsburg, Alexandre A. Differential ion mobility separations/mass spectrometry with high resolution in both dimensions. Anal. Chem., 2019, 91 (2), pp 1479–1485
Abstract

Strong orthogonality to mass spectrometry makes differential ion mobility spectrometry (FAIMS) a powerful tool for isomer separations. However, high FAIMS resolution has been achieved overall only with buffers rich in He or H2. That obstructed coupling to Fourier transform mass spectrometers operating under ultrahigh vacuum, but exceptional m/z resolution and accuracy of FTMS are indispensable for frontline biological and environmental applications. By raising the waveform amplitude to 6 kV, we enabled high FAIMS resolution using solely N2 and thus straightforward integration with any MS platform: here Orbitrap XL with the electron transfer dissociation (ETD) option. The initial evaluation for complete histone tails (50 residues) with diverse post-translational modifications on alternative sites demonstrates a broad capability to separate and confidently identify the PTM localization variants in the middle-down range.

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Publisher
American Chemical Society
Journal
Book Title
Series
Analytical Chemistry;v.91:no.2
PubMed ID
DOI
ISSN
0003-2700
EISSN