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dc.contributor.authorHurley, Matthew T.
dc.contributor.authorWang, Zifan
dc.contributor.authorMahle, Amanda
dc.contributor.authorRabin, Daniel
dc.contributor.authorLiu, Qing
dc.contributor.authorEnglish, Douglas S.
dc.contributor.authorZachariah, Michael R.
dc.contributor.authorStein, Daniel
dc.contributor.authorDeShong, Philip
dc.identifier.citationHurley, Matthew T.; Wang, Zifan; Mahle, Amanda; Rabin, Daniel; Liu, Qing; English, Douglas S.; Zachariah, Michael R.; Stein, Daniel; DeShong, Philip. 2013. Synthesis, characterization, and application of antibody functionalized fluorescent silica nanoparticles. Advanced Functional Materials, vol. 23:no. 26:ppg. 3335-3343en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractFluorescent silica nanoparticles (FSNs) are prepared by incorporating dye into a mesoporous silica nanoparticle (MSN) synthesis procedure. FSNs containing sulforhodamine B, hydrophobically modified sulforhodamine B, and Cascade Blue hydrazide are made. The MSN-based FSNs do not leach dye under simulated physiological conditions and have strong, stable fluorescence. FSNs prepared with sulforhodamine B are compared to FSNs prepared with hydrophobically modified sulforhodamine B. The data indicate that FSNs prepared with sulforhodamine B are equally as stable but twice as fluorescent as particles made with hydrophobically modified sulforhodamine B. The fluorescence of a FSN prepared with sulforhodamine B is 10 times more intense than the fluorescence of a 4.5 nm core-shell CdSe/ZnS quantum dot. For diagnostic applications, a method to selectively and covalently bind antibodies to the surface of the FSNs is devised. FSNs that are functionalized with antibodies specific for Neisseria gonorrhoeae specifically bind to Neisseria gonorrhoeae in flow cytometry experiments, thus demonstrating the functionality of the attached antibodies and the potential of MSN-based FSNs to be used in diagnostic applications.en_US
dc.description.sponsorshipNational Science Foundation (NIRT, CHE 0511219478, "Stimuli-Response Hybrid Nanoparticles for Controlled Chemical Delivery"), the Maryland Technology Development Corporation, and SD Nanosciences, Inc. We gratefully acknowledge the support of the Maryland NanoCenter and its NispLab. The NispLab is supported in part by the NSF as a MRSEC Shared Experimental Facility. M.T.H. acknowledges the support of the Graduate Assistance in Areas of National Need (GAANN) Fellowship. D.C.S. acknowledges the support from a grant from the National Institutes of Health (AI068888). A.M. was supported by a T32 training grant from the National Institutes of Health (AI09621).en_US
dc.publisherWiley-V C H Verlag GMBHen_US
dc.relation.ispartofseriesAdvanced Functional Materials;v.23:no.26
dc.subjectFluorescent silica nanoparticlesen_US
dc.subjectFluorescence microscopyen_US
dc.subjectAntibody functionalized silica nanoparticlesen_US
dc.subjectFlow cytometryen_US
dc.titleSynthesis, characterization, and application of antibody functionalized fluorescent silica nanoparticlesen_US

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