• Login
    View Item 
    •   Shocker Open Access Repository Home
    • Graduate Student Research
    • ETD: Electronic Theses and Dissertations
    • Master's Theses
    • View Item
    •   Shocker Open Access Repository Home
    • Graduate Student Research
    • ETD: Electronic Theses and Dissertations
    • Master's Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Fabrication and characterization of antibacterial Polycaprolactone and natural Hydroxyapatite nanofibers for bone tissue scaffolds

    View/Open
    t13035_Patrick.pdf (2.623Mb)
    Date
    2013-05
    Author
    Patrick, Stephanie Marie
    Advisor
    Asmatulu, Ramazan
    Metadata
    Show full item record
    Abstract
    Chronic osteomyelitis is a bone infection that may result in pain, pus, bone resorption and damage, and fractures. The disease often needs prolonged antibiotic therapy, and in many cases severe wounds and bone voids are caused by surgical interventions. Autograft, allograft, xenograft, or synthetic materials have been used as bone fillers or scaffolds. Gentamicin is a common antibiotic in osteomyelitis treatment; including gentamicin in the scaffold therefore would help treat the osteomyelitis once the scaffold is in place and help prevent spreading of the disease. Hydroxyapatite (HA) is a mineral that is naturally found in bone that has osteoconductive properties in bone tissue engineering. I hypothesize that a bone graft substitute incorporating both gentamicin and HA would be very beneficial for the treatment of osteomyelitis with large bone damage. While there are many methods to fabricate porous graft using a biodegradable polymer, electrospinning technique is particularly ideal due to nano-fibrous structure resembling the extracellular matrix of bone. The objectives of my thesis work are to develop a gentamicin-contained PCL-HA composite scaffold and to evaluate its therapeutic efficacy in inhibiting E. coli growth using at in vitro settings. PCL-HA composite nanofibers were fabricated using electrospinning with inclusions of gentamicin to give the nanofibers antibacterial properties. HA was obtained from cow bone, with SEM and EDS examinations confirming that its chemical structure and size were well suited to promote bone growth. SEM micrographs illustrated the nano-scaled fiber structures with an average diameter of 142.2 nm, and biological tests revealed that the gentamicin-containing PCL-HA nano-fiber membranes effectively exterminated E. coli's growth up to 7 days, with zones of inhibition to 4 cm2. Further study is warranted to characterize the antibiotic release patterns in vivo and the potential safety issues.
    Description
    Thesis (M.S.)--Wichita State University, College of Engineering, Dept. of Mechanical Engineering
    URI
    http://hdl.handle.net/10057/6833
    Collections
    • CE Theses and Dissertations
    • Master's Theses
    • ME Theses and Dissertations

    Browse

    All of Shocker Open Access RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsBy TypeThis CollectionBy Issue DateAuthorsTitlesSubjectsBy Type

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV