Gene delivery with PCL nanofiber in vitro
AdvisorAsmatulu, Ramazan; Yang, Shang-You
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Ceylan, Muhammet and Jianhao Jiang. 2013. Gene Delivery with PCL Nanofiber in Vitro. -- In Proceedings: 9th Annual Symposium: Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p.24-25
Gene therapy has widely been known to be the promising treatment approach for many diseases, whether acquired or inherited through a genetic disorder. Viral and nonviral vectors are the primary research fields of gene delivery techniques which play a pivotal role in tissue engineering and cancer therapy. Even though viral vectors are well-known as higher transfection efficiency, they are also carefully applied in human because of their side effects. Non-viral vectors, such as synthetic and natural compounds and polymers which have less toxicity and low immunogenicity, can offer flexible choice of gene to deliver. However, there are some obstacles in non-viral gene delivery, including low gene transfection efficiency and sudden release. In this study, Polycaprolactone (PCL) and plasmid DNA was dissolved in acetonitrile and electrospun. Our study showed that the transfection efficiency was improved under the controlled gene delivery release in vitro condition.
Paper presented to the 9th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Marcus Welcome Center, Wichita State University, May 8, 2013.
Research completed at the Department of Mechanical Engineering, College of Engineering, and the Department of Biological Sciences, College of Arts and Sciences