Gene delivery with PCL nanofiber in vitro

Abstract

Gene therapy has widely been known to be the promising treatment approach for many diseases, whether acquired or inherited through a genetic disorder. Viral and nonviral vectors are the primary research fields of gene delivery techniques which play a pivotal role in tissue engineering and cancer therapy. Even though viral vectors are well-known as higher transfection efficiency, they are also carefully applied in human because of their side effects. Non-viral vectors, such as synthetic and natural compounds and polymers which have less toxicity and low immunogenicity, can offer flexible choice of gene to deliver. However, there are some obstacles in non-viral gene delivery, including low gene transfection efficiency and sudden release. In this study, Polycaprolactone (PCL) and plasmid DNA was dissolved in acetonitrile and electrospun. Our study showed that the transfection efficiency was improved under the controlled gene delivery release in vitro condition.