Show simple item record

dc.contributor.authorJiang, Yunpeng
dc.contributor.authorJia, Tanghong
dc.contributor.authorGong, Weiming
dc.contributor.authorWooley, Paul H.
dc.contributor.authorYang, Shang-You
dc.date.accessioned2013-09-23T18:47:52Z
dc.date.available2013-09-23T18:47:52Z
dc.date.issued2013-10
dc.identifier.citationJiang Y, Jia T, Gong W, Wooley PH, Yang S.-Y. 2013. Effects of Ti, PMMA, UHMWPE, and Co-Cr wear particles on differentiation and functions of bone marrow stromal cells. J Biomedical Material Research Part A, v.101:no.10:pp.2817-2825en_US
dc.identifier.issn1549-3296
dc.identifier.otherWOS:000323648300007
dc.identifier.urihttp://dx.doi.org/10.1002/jbm.a.34595
dc.identifier.urihttp://hdl.handle.net/10057/6498
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractThis study investigates the roles of orthopedic biomaterial particles [Ti-alloy, poly(methyl methacrylate) (PMMA), ultrahigh-molecular-weight polyethylene (UHMWPE), Co-Cr alloy] on the differentiation and functions of bone marrow stromal cells (BMSCs). Cells were isolated from femurs of BALB/c mice and cultured in complete osteoblast-induction medium in presence of micron-sized biomaterial particles at various doses. 3-(4,5)-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and lactate dehydrogenase assay were performed for cell proliferation and cytotoxicity. Differentiation and function of osteoblasts were evaluated by alkaline phosphatase (ALP), osteocalcin, RANKL, OSX, and Runx2 expressions. Murine interleukin-1 (IL-1), IL-6, and tumor necrosis factor- in culture media were determined by enzyme-linked immunosorbent assay. Challenge with low doses of Ti, UHMWPE, or Co-Cr particles markedly promoted the bone marrow cell proliferation while high dose of Co-Cr significantly inhibited cell growth (p < 0.05). Cells challenged with low dose of PMMA or UHMWPE particles (0.63 mg/mL) exhibited strong ALP activity, whereas Ti and Co-Cr groups showed minimal effects (p < 0.05). UHMWPE and Ti particles also promoted higher expression of proinflammatory cytokines. Real-time polymerase chain reaction data suggested that cells treated with low dose (0.5 mg/mL) particles resulted in distinctly diminished RANKL expression compared to those exposed to high concentrated (3 mg/mL) particles. In conclusion, various types of wear debris particles behaved differently in the differentiation, maturation, and functions of osteogenic cells; and the particulate debris-interacted BMSCs may play an important role in the pathogenesis and process of the debris-associated aseptic prosthetic loosening.en_US
dc.description.sponsorshipNational Institutes of Health; contract grant number: 5R03AR054929 (S-YY) and the Orthopaedic Research Institute, Via Christi Healthen_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley and Sonsen_US
dc.relation.ispartofseriesJournal of Biomedical Materials Research Part A;v.101:no.10
dc.subjectaseptic looseningen_US
dc.subjectwear debrisen_US
dc.subjectosteoblasten_US
dc.subjectbone marrow stromal cellsen_US
dc.subjectgene expressionen_US
dc.titleEffects of Ti, PMMA, UHMWPE, and Co-Cr wear particles on differentiation and functions of bone marrow stromal cellsen_US
dc.typeArticleen_US
dc.description.versionPeer reviewed
dc.rights.holderCopyright © 2013 Wiley Periodicals, Inc., a Wiley Company


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record