Are SH-SY5Y and MN9D cell lines truly dopaminergic?
Citation
Balasooriya, Inoka S. & Wimalasena, Kandatage . (2007). Are SH-SY5Y and MN9D cell lines truly dopaminergic?. In Proceedings : 3rd Annual Symposium : Graduate Research and Scholarly Projects. Wichita, KS : Wichita State University, p.25-26
Abstract
SH-SY5Y and MN9D cell lines are commonly
used dopaminergic models in studies related to neurotoxicity,
oxidative stress, and neurodegenerative diseases. Early studies
suggested that SH-SY5Y cells do not convert intracellular DA
to NE even though high levels of DβM are present in these
cells. In contrast, our studies show that these cells do not
store substantial levels of DA or NE, but extracellular DA is
taken up and converted to NE efficiently. The efficiency of
DA uptake and its conversion to NE increase with the number
of cell passages. Kinetic studies show that NE is a better
substrate for plasma membrane transporters than DA. In
contrast to undifferentiated cells, 12-O-tetradecanoyl-phorbol-
13-acetate (TPA) differentiated SH-SY5Y cells store
substantially higher levels of NE. These cells take up DA and
NE more efficiently than undifferentiated cells. Therefore,
differentiated and undifferentiated high passage SH-SY5Y
cells could be used as a noradrenergic, but not as a
dopaminergic model. MN9D cells store high levels of DA
under normal growth conditions, but do not convert DA to
NE. They show poor catecholamine uptake characteristics
compared to undifferentiated SH-SY5Y cells, however nbutyric
acid differentiated MN9D cells show efficient DA
uptake kinetics similar to undifferentiated SH-SY5Y,
suggesting that they could be used as a reasonable
dopaminergic model.
Description
Paper presented to the 3rd Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Hughes Metropolitan Complex, Wichita State University, April 27, 2007.
Research completed at the Department of Chemistry, College of Liberal Arts and Sciences