Show simple item record

dc.contributor.authorRobert, Philippe
dc.contributor.authorTroalen, Frédéric
dc.contributor.authorBellet, Dominique
dc.contributor.authorBousfield, George R.
dc.contributor.authorBidart, Jean-Michel
dc.date.accessioned2013-07-22T20:13:52Z
dc.date.available2013-07-22T20:13:52Z
dc.date.issued1994-05
dc.identifier.citationPhilippe Robert, Frédéric Troalen, Dominique Bellet, Georges R. Bousfield, Jean-Michel Bidart, Immunochemical mapping of human lutropin: I. Delineation of a conformational antigenic determinant, Molecular and Cellular Endocrinology, Volume 101, Issues 1–2, May 1994, Pages 11-20, ISSN 0303-7207, http://dx.doi.org/10.1016/0303-7207(94)90214-3. (http://www.sciencedirect.com/science/article/pii/0303720794902143)en_US
dc.identifier.issn0303-7207 (Print)
dc.identifier.issn1872-8057 (Electronic)
dc.identifier.urihttp://dx.doi.org/10.1016/0303-7207(94)90214-3
dc.identifier.urihttp://hdl.handle.net/10057/6012
dc.descriptionClick on the DOI link to access this article (may not be free)en_US
dc.description.abstractLutropin (LH), follitropin (FSH), thyrotropin (TSH) and choriogonadotropin (CG) are assembled of two non-covalently alpha (α) and beta (β) subunits. We studied the discontinuous antigenic regions recognized by a monoclonal anti-hLH antibody designated as LH05 which binds to hLH, hCG and hTSH and does not cross-react with either the free subunits or hFSH. LH05 and an antibody designated HT13, recognizing an epitope partly comprizing the α64–76 region, did not bind simultaneously to hCG. Furthermore, LH05 was unable to combine with an anti-peptide antibody (LHP03) directed to residues 43–52 of hLHβ. Thus, LH05 recognizes an epitope partly overlapping with those recognized by HT13 and LHP03. Using various hybrid molecules, we showed that the human α-subunit plays a critical role in the assembly of the epitope that, in contrast, contains amino acid residues conserved in the various β-subunit of several species. Together, our results suggest that the amino acids Leu49-Pro50, Tyr59-Arg60 and Leu86-Ser87 in the hLHβ and the α64–76 region are probably included in the epitope recognizedby LH05 which appeared to be not accessible on the CG/LH receptor.en_US
dc.language.isoen_USen_US
dc.publisherElsevier Ireland Ltd.en_US
dc.relation.ispartofseriesMolecular and Cellular Endocrinology;
dc.relation.ispartofseries;V.101, No.1-2
dc.subjectLutropinen_US
dc.subjectMonoclonal antibodyen_US
dc.subjectStructureen_US
dc.subjectSubuniten_US
dc.titleImmunochemical mapping of human lutropin: I. Delineation of a conformational antigenic determinanten_US
dc.typeArticleen_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record