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dc.contributor.authorOduru, Sreedhar
dc.contributor.authorCampbell, Janee L.
dc.contributor.authorKarri, SriTulasi
dc.contributor.authorHendry, William J. III
dc.contributor.authorKhan, Shafiq A.
dc.contributor.authorWilliams, Simon C.
dc.date.accessioned2013-07-15T19:59:51Z
dc.date.available2013-07-15T19:59:51Z
dc.date.issued2003-06-03
dc.identifier.citationOduru S, Campbell JL, Karri S, Hendry WJ, Khan SA, Williams SC; Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs; BMC Genomics. 2003 Jun 3;4(1):22. Epub 2003 Jun 3; doi:10.1186/1471-2164-4-22; http://www.biomedcentral.com/1471-2164/4/22en_US
dc.identifier.issn1471-2164 (Print)
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-4-22
dc.identifier.urihttp://hdl.handle.net/10057/5971
dc.description© 2003 Oduru et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL (http://www.biomedcentral.com/1471-2164/4/22)en_US
dc.description.abstractBackground Complete genome annotation will likely be achieved through a combination of computer-based analysis of available genome sequences combined with direct experimental characterization of expressed regions of individual genomes. We have utilized a comparative genomics approach involving the sequencing of randomly selected hamster testis cDNAs to begin to identify genes not previously annotated on the human, mouse, rat and Fugu (pufferfish) genomes. Results 735 distinct sequences were analyzed for their relatedness to known sequences in public databases. Eight of these sequences were derived from previously unidentified genes and expression of these genes in testis was confirmed by Northern blotting. The genomic locations of each sequence were mapped in human, mouse, rat and pufferfish, where applicable, and the structure of their cognate genes was derived using computer-based predictions, genomic comparisons and analysis of uncharacterized cDNA sequences from human and macaque. Conclusion The use of a comparative genomics approach resulted in the identification of eight cDNAs that correspond to previously uncharacterized genes in the human genome. The proteins encoded by these genes included a new member of the kinesin superfamily, a SET/MYND-domain protein, and six proteins for which no specific function could be predicted. Each gene was expressed primarily in testis, suggesting that they may play roles in the development and/or function of testicular cells.en_US
dc.language.isoen_USen_US
dc.publisherOduru et alen_US
dc.relation.ispartofseriesBMC Genomics;
dc.relation.ispartofseries;V.4, No.1
dc.titleGene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAsen_US
dc.typeArticleen_US


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