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dc.contributor.authorKim, Yunjeong
dc.contributor.authorLovell, Scott
dc.contributor.authorTiew, Kok-Chuan
dc.contributor.authorMandadapu, Sivakoteswara Rao
dc.contributor.authorAlliston, Kevin R.
dc.contributor.authorBattaile, Kevin P.
dc.contributor.authorGroutas, William C.
dc.contributor.authorChang, Kyeong-Ok
dc.identifier.citationKim, Yunjeong; Lovell, Scott; Tiew, Kok-Chuan; Mandadapu, Sivakoteswara Rao; Alliston, Kevin R.; Battaile, Kevin P.; Groutas, William C.; Chang, Kyeong-Ok. 2012. Broad-spectrum antivirals against 3C or 3C-like proteases of picornaviruses, noroviruses, and coronaviruses. Journal of Virology, v.86 no.21 pp.11754-11762en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractPhylogenetic analysis has demonstrated that some positive-sense RNA viruses can be classified into the picornavirus-like supercluster, which includes picornaviruses, caliciviruses, and coronaviruses. These viruses possess 3C or 3C-like proteases (3Cpro or 3CLpro, respectively), which contain a typical chymotrypsin-like fold and a catalytic triad (or dyad) with a Cys residue as a nucleophile. The conserved key sites of 3Cpro or 3CLpro may serve as attractive targets for the design of broad-spectrum antivirals for multiple viruses in the supercluster. We previously reported the structure-based design and synthesis of potent protease inhibitors of Norwalk virus (NV), a member of the Caliciviridae family. We report herein the broad-spectrum antiviral activities of three compounds possessing a common dipeptidyl residue with different warheads, i.e., an aldehyde (GC373), a bisulfite adduct (GC376), and an α-ketoamide (GC375), against viruses that belong to the supercluster. All compounds were highly effective against the majority of tested viruses, with half-maximal inhibitory concentrations in the high nanomolar or low micromolar range in enzyme- and/or cell-based assays and with high therapeutic indices. We also report the high-resolution X-ray cocrystal structures of NV 3CLpro-, poliovirus 3Cpro-, and transmissible gastroenteritis virus 3CLpro- GC376 inhibitor complexes, which show the compound covalently bound to a nucleophilic Cys residue in the catalytic site of the corresponding protease. We conclude that these compounds have the potential to be developed as antiviral therapeutics aimed at a single virus or multiple viruses in the picornavirus-like supercluster by targeting 3Cpro or 3CLpro.en_US
dc.publisherAmerican Society of Microbiologyen_US
dc.relation.ispartofseriesJournal of Virology;v.86 no.21
dc.subjectStructure-based designen_US
dc.subjectBiological evaluationen_US
dc.subjectBinding siteen_US
dc.subjectHuman reninen_US
dc.titleBroad-spectrum antivirals against 3C or 3C-like proteases of picornaviruses, noroviruses, and coronavirusesen_US
dc.description.versionPeer reviewed

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