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dc.contributor.authorYu, Haiying
dc.contributor.authorWooley, Paul H.
dc.contributor.authorYang, Shang-You
dc.date.accessioned2013-07-08T19:38:25Z
dc.date.available2013-07-08T19:38:25Z
dc.date.issued2009-02-26
dc.identifier.citationHaiying Yu, Paul H Wooley, Shang-You Yang; Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells; Journal of Orthopaedic Surgery and Research Feb.2009, V.4, No.5;en_US
dc.identifier.issn1749-799X
dc.identifier.urihttp://dx.doi.org/ 10.1186/1749-799X-4-5
dc.identifier.urihttp://hdl.handle.net/10057/5883
dc.descriptionOpen Access. Click on the DOI link to access this article at the publisher's website, or download /view it on SOAR.en_US
dc.description.abstractBackground. Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-ε-caprolactone (PCL) composite scaffolds. Methods Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel™ for endothelial cells. Both types of cell were seeded respectively on PCL-HA scaffolds at HA to PCL weight ratio of 1:1, 1:4, or 0:1 and were evaluated using scanning electron microscopy, ALP activity (of osteoblasts) and nitric oxide production (of endothelial cells) plus the assessment of cell viability. Results The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability. An elevated nitric oxide production of endothelial cells was observed in HA-containing group. Conclusion Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells. The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.ispartofseriesJournal of Orthopaedic Surgery and Research;
dc.relation.ispartofseries;V.4, No.5
dc.rights.urihttp://www.josr-online.com/
dc.titleBiocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cellsen_US
dc.typeArticleen_US
dc.description.versionPeer reviewed
dc.rights.holder© 2009 Yu et al; licensee BioMed Central Ltd.


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