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dc.contributor.authorWang, Zifan
dc.contributor.authorGoodson, Kathy A.
dc.contributor.authorHaeusler, Aaron R.
dc.contributor.authorEnglish, Douglas S.
dc.contributor.authorKahn, Jason D.
dc.date.accessioned2013-06-26T17:18:14Z
dc.date.available2013-06-26T17:18:14Z
dc.date.issued2012-04-18
dc.identifier.citationZifan Wang, Kathy Goodson, Aaron R. Haeusler, Doug English, Jason D. Kahn. (2012). LacI-DNA-IPTG Loops: Equilibria among Conformations by Single-Molecule FRET. -- In Proceedings: 8th Annual Symposium: Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p.147-148en_US
dc.identifier.urihttp://hdl.handle.net/10057/5812
dc.descriptionPaper presented to the 8th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Marcus Welcome Center, Wichita State University, April 18, 2012.
dc.descriptionResearch completed at Wichita State University, Wichita, KS; University of Maryland, College Park, MD
dc.description.abstractThe E. coli Lac repressor (LacI) tetramer binds simultaneously to a promoter-proximal DNA binding site (operator) and an auxiliary operator, resulting in a DNA loop, which increases repression efficiency. Induction of the lac operon by allolactose reduces the affinity of LacI for DNA, but induction does not completely prevent looping in vivo. Single molecule fluorescence resonance energy transfer (SM-FRET) on a dual fluorophore-labeled LacI-9C14 loop showed that it adopts a single, stable, high-FRET V-shaped LacI conformation. Ligand-induced changes in loop geometry can affect loop stability. SM-FRET confirms that the high-FRET LacI-9C14 loop is only partially destabilized by saturating IPTG. FRET histograms suggest that the remaining population is a mixture of lower-FRET states ascribed to specific nonspecific or extended LacI loops, not free DNA.en_US
dc.description.sponsorshipGraduate School, Office of Research Administration, University Librariesen_US
dc.language.isoen_USen_US
dc.publisherWichita State University. Graduate School.en_US
dc.relation.ispartofseriesGRASP;
dc.relation.ispartofseriesv.8;
dc.titleLacI-DNA-IPTG loops: Equilibria among conformations by single-molecule FRETen_US
dc.typeConference paper
dc.rights.holderWichita State University


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