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dc.contributor.authorNing, Bin
dc.contributor.authorLiu, Haifei
dc.contributor.authorGong, Weiming
dc.contributor.authorJiang, Jianhao
dc.contributor.authorHu, Yougu
dc.contributor.authorYang, Shang-You
dc.date.accessioned2013-06-25T16:01:01Z
dc.date.available2013-06-25T16:01:01Z
dc.date.issued2013-06
dc.identifier.citationNing, B., Liu, H., Gong, W., Jiang, J., Hu, Y. and Yang, S.-Y. (2013), Biological characteristics of adult degenerative nucleus pulposus cells in a three-dimensional microcarrier stirring culture system. J. Orthop. Res., 31: 858–863. doi: 10.1002/jor.22306en_US
dc.identifier.issn0736-0266
dc.identifier.urihttp://dx.doi.org/10.1002/jor.22306
dc.identifier.urihttp://hdl.handle.net/10057/5761
dc.descriptionClick on the DOI link to access the article (may not be free)en_US
dc.description.abstractA major problem in reconstructing degenerative intervertebral discs is to obtain sufficient nucleus pulposus (NP) seeding cells with normal physiologic functions. The current study adopted a three-dimensional microcarrier culture system for massive cell expansion and evaluated the biological characteristics and physiological functions of the propagated adult degenerative NP cells. Isolated adult NP cells were cultured in either microcarrier stirring culturing system or traditional monolayer cultivation. The growth characteristics, proliferation, extracellular matrix secretion, and apoptosis potential were examined to evaluate the different features of the two cultivation methods. Compared to the monolayer cultivation system, the adhesion time of NP cells in the three-dimensional microcarrier culture system appeared longer with relatively transient stable growth period. MTT and 3H-TdR assays suggested significantly elevated proliferation and higher thymidine incorporation rates in cells from microcarrier system compare to cells in the monolayer system at the exponential growth phase (p < 0.05). Western blot data complimented the immunostaining results that the NP cells in the microcarrier system expressed significantly more protein levels of both type collagens at the exponential growth phase than that in the monolayer system (p < 0.05). Further, significantly more 35S labeled proteoglycan incorporation was noticed in the cells on the microcarriers at both the stable growth and the exponential growth phases (p < 0.05 and p < 0.01). In conclusion, the three-dimensional microcarrier stirring culture system provides a means of fast and massive propagation of NP seeding cells which maintain their normal physiological characteristics and functions.en_US
dc.description.sponsorshipNational Natural Science Foundation of P.R. China. Grant Numbers: 30271318, 30672132en_US
dc.language.isoen_USen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofseriesJournal of Orthopaedic Research;v.31, no.6
dc.subjectNucleus pulposusen_US
dc.subjectMicrocarrier cultureen_US
dc.subjectCollagensen_US
dc.subjectProteoglycanen_US
dc.titleBiological characteristics of adult degenerative nucleus pulposus cells in a three-dimensional microcarrier stirring culture systemen_US
dc.typeArticleen_US
dc.description.versionPeer reviewed
dc.rights.holderCopyright © 2013 Orthopaedic Research Society


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