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dc.contributor.authorHerron, Bruce J.
dc.contributor.authorLu, Weining
dc.contributor.authorRao, Cherie
dc.contributor.authorLiu, Shanming
dc.contributor.authorPeters, Heiko
dc.contributor.authorBronson, Roderick T.
dc.contributor.authorJustice, Monica J.
dc.contributor.authorMcDonald, J. David
dc.contributor.authorBeier, David R.
dc.identifier.citationHerron, Bruce J.; Lu, Weining; Rao, Cherie; Liu, Shanming; Peters, Heiko; Bronson, Roderick T.; Justice, Monica J.; McDonald, J. David; Beier, David R. 2002. Efficient generation and mapping of recessive developmental mutations using ENU mutagenesis. Nature Genetics, v.30 no.2 pp.185-189en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractTreatment with N-ethyl-N-nitrosourea (ENU) efficiently generates single-nucleotide mutations in mice. Along with the renewed interest in this approach, much attention has been given recently to large screens with broad aims; however, more finely focused studies have proven very productive as well. Here we show how mutagenesis together with genetic mapping can facilitate the rapid characterization of recessive loci required for normal embryonic development. We screened third-generation progeny of mutagenized mice at embryonic day (E) 18.5 for abnormalities of organogenesis. We ascertained 15 monogenic mutations in the 54 families that were comprehensively analyzed. We carried out the experiment as an outcross, which facilitated the genetic mapping of the mutations by haplotype analysis. We mapped seven of the mutations and identified the affected locus in two lines. Using a hierarchical approach, it is possible to maximize the efficiency of this analysis so that it can be carried out easily with modest infrastructure and resources.en_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseriesNature Genetics;v.30 no.2
dc.titleEfficient generation and mapping of recessive developmental mutations using ENU mutagenesisen_US

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