• Login
    View Item 
    •   Shocker Open Access Repository Home
    • Fairmount College of Liberal Arts and Sciences
    • Biological Sciences
    • BIO Faculty Scholarship
    • BIO Faculty Publications
    • View Item
    •   Shocker Open Access Repository Home
    • Fairmount College of Liberal Arts and Sciences
    • Biological Sciences
    • BIO Faculty Scholarship
    • BIO Faculty Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Recovery and STR amplification of DNA from RFLP membranes

    Date
    2008-03-24
    Author
    Steadman, Shelly A.
    McDonald, J. David
    Andrews, John S.
    Watson, Nigel D.
    Metadata
    Show full item record
    Citation
    Steadman, Shelly A.; McDonald, J. David; Andrews, John S.; Watson, Nigel D. 2008. Recovery and STR amplification of DNA from RFLP membranes. Journal of Forensic Sciences, v.53 no.2 pp.349-358
    Abstract
    Restriction fragment length polymorphism (RFLP) techniques were utilized in the forensic DNA community until the mid 1990s when less labor-intensive polymerase chain reaction short tandem repeat (PCR STR) techniques became available. During the transition from RFLP technology to PCR-based STR platforms, a method for comparing RFLP profiles to STR profiles was not developed. While the preferred approach for applying new technology to old cases would be to analyze the original biological stain, this is not always possible. For unsolved cases that previously underwent RFLP analysis, the only DNA remaining may be restriction cut and bound to nylon membranes. These studies investigate several methods for obtaining STR profiles from membrane bound DNA, including removal of bound DNA with bases, acids, detergents, various chemicals, and conventional cell extraction solutions. Direct multiplex STR amplification of template in the membrane-bound state was also explored. A partial STR profile was obtained from DNA that was recovered from an archived membrane using conventional extraction buffer components, indicating promise for recovering useful STR information from RFLP membranes that have been maintained in long-term frozen storage.
    Description
    Click on the DOI link to access the article (may not be free).
    URI
    http://dx.doi.org/10.1111/j.1556-4029.2008.00674.x
    http://hdl.handle.net/10057/5629
    Collections
    • BIO Faculty Publications

    Browse

    All of Shocker Open Access RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsBy TypeThis CollectionBy Issue DateAuthorsTitlesSubjectsBy Type

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DSpace software copyright © 2002-2021  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV