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dc.contributor.authorKim, Yunjeong
dc.contributor.authorMandadapu, Sivakoteswara Rao
dc.contributor.authorGroutas, William C.
dc.contributor.authorChang, Kyeong-Ok
dc.date.accessioned2013-02-09T23:54:12Z
dc.date.available2013-02-09T23:54:12Z
dc.date.issued2012-11-28
dc.identifier.citationKim, Yunjeong; Mandadapu, Sivakoteswara Rao; Groutas, William C.; Chang, Kyeong-Ok. 2012. Potent inhibition of feline coronaviruses with peptidyl compounds targeting coronavirus 3C-like protease. Antiviral Research, v. 97, issue 2 (February 2013), pp.161–168en_US
dc.identifier.issn0166-3542
dc.identifier.urihttp://dx.doi.org/10.1016/j.antiviral.2012.11.005
dc.identifier.urihttp://hdl.handle.net/10057/5513
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractFeline coronavirus infection is common among domestic and exotic felid species and usually associated with mild or asymptomatic enteritis; however, feline infectious peritonitis (FIP) is a fatal disease of cats that is caused by systemic infection with a feline infectious peritonitis virus (FIPV), a variant of feline enteric coronavirus (FECV). Currently, there is no specific treatment approved for FIP despite the importance of FIP as the leading infectious cause of death in young cats. During the replication process, coronavirus produces viral polyproteins that are processed into mature proteins by viral proteases, the main protease (3C-like [3CL] protease) and the papain-like protease. Since the cleavages of viral polyproteins are an essential step for virus replication, blockage of viral protease is an attractive target for therapeutic intervention. Previously, we reported the generation of broad-spectrum peptidyl inhibitors against viruses that possess a 3C or 3CL protease. In this study, we further evaluated the antiviral effects of the peptidyl inhibitors against feline coronaviruses, and investigated the interaction between our protease inhibitor and a cathepsin B inhibitor, an entry blocker, against feline coronaviruses in cell culture. Herein we report that our compounds behave as reversible, competitive inhibitors of 3CL protease, potently inhibited the replication of feline coronaviruses (EC50 in a nanomolar range) and, furthermore, the combination of cathepsin B and 3CL protease inhibitors led to a strong synergistic interaction against feline coronaviruses in cell culture systems.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesAntiviral Research
dc.relation.ispartofseriesv.97, no.2
dc.subjectFeline coronavirusesen_US
dc.subjectFeline infectious peritonitis virusen_US
dc.subjectProtease inhibitoren_US
dc.subjectCathepsin Ben_US
dc.subjectSynergyen_US
dc.subject3CL proteaseen_US
dc.titlePotent inhibition of feline coronaviruses with peptidyl compounds targeting coronavirus 3C-like proteaseen_US
dc.typeArticleen_US
dc.description.versionPeer reviewed
dc.rights.holderCopyright © 2012 Elsevier B.V. All rights reserved.


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