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dc.contributor.authorEns, S.
dc.contributor.authorOlson, A. B.
dc.contributor.authorDudley, Chris J.
dc.contributor.authorRoss III, N.D.
dc.contributor.authorSiddiqi, A. A.
dc.contributor.authorUmoh, K. M.
dc.contributor.authorSchneegurt, Mark A.
dc.identifier.citationEns, S., Olson, A. B., Dudley, C., Ross, N. D., Siddiqi, A. A., Umoh, K. M. and Schneegurt, M. A. (2012), Inexpensive and safe DNA gel electrophoresis using household materials. Biochem. Mol. Biol. Educ., 40: 198–203. doi: 10.1002/bmb.20596en_US
dc.identifier.otherWOS: 000304303000007
dc.descriptionClick on the DOI link below to access the article (may not be free).en_US
dc.description.abstractGel electrophoresis is the single most important molecular biology technique and it is central to life sciences research, but it is often too expensive for the secondary science classroom or homeschoolers. A simple safe low-cost procedure is described here that uses household materials to construct and run DNA gel electrophoresis. Plastic containers are fitted with aluminum foil electrodes and 9-V batteries to run food-grade agar-agar gels using aquarium pH buffers and then stained with gentian violet. This activity was tested in a high school biology classroom with significantly positive responses on postactivity reflective surveys. The electrophoresis activity addresses several Life Science Content Standard C criteria, including aspects of cell biology, genetics, and evolution. It also can be used to teach aspects of motion and force in the physical science classroom.en_US
dc.description.sponsorshipThis work is supported by the National Science Foundation Graduate STEM Fellows in K-12 Education (GK-12) program and the Watkins Foundation of Wichita through the Watkins Summer Research Participation Fellowships for Teachers of Science and from the Wichita Public Schools (USD259).en_US
dc.relation.ispartofseriesBiochemistry and Molecular Biology Education;2012, v.40, no.3
dc.subjectMolecular biologyen_US
dc.subjectHigh schoolen_US
dc.subjectLaboratory exercisesen_US
dc.subjectPhysical sciencesen_US
dc.subject.classificationBIOCHEMISTRY & MOLECULAR BIOLOGY
dc.subject.classificationEDUCATION & EDUCATIONAL RESEARCH
dc.titleInexpensive and safe DNA gel electrophoresis using household materialsen_US
dc.rights.holderCopyright 2012 by The International Union of Biochemistry and Molecular Biology

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