dc.contributor | Wichita State University. Department of Chemistry | en_US |
dc.contributor.author | Otim, Ochan | en_US |
dc.contributor.author | Singhal, Ram P. | en_US |
dc.date.accessioned | 2012-02-06T17:17:18Z | |
dc.date.available | 2012-02-06T17:17:18Z | |
dc.date.issued | 1997-09-01 | en_US |
dc.identifier | 9725123 | en_US |
dc.identifier | 9507610 | en_US |
dc.identifier.citation | Journal of capillary electrophoresis. 1997 Sep-Oct; 4(5): 247-51. | en_US |
dc.identifier.issn | 1079-5383 | en_US |
dc.identifier.uri | http://hdl.handle.net/10057/4407 | |
dc.description | Full text of this article is not available in SOAR. | en_US |
dc.description.abstract | The electrophoretic resolution (selectivity) of double-stranded DNA (dsDNA) restriction fragments is studied for different organisms (phi X174, lambda phage, and pGEM-3 plasmids) by various separation and detection methods. The selectivity can be predicted by plotting the difference in chain length over their average chain length (base pair difference/average base pair magnitude of delta bp/Ave.-bp) against each DNA fragment pair. Experimental conditions and the nature of the DNA can influence the predicted results. | en_US |
dc.format.extent | 247-51 | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ISC Technical Publications | en_US |
dc.relation.ispartofseries | Journal of capillary electrophoresis | en_US |
dc.relation.ispartofseries | J Capillary Electrophor | en_US |
dc.source | NLM | en_US |
dc.subject | Research Support, U.S. Gov't, Non-P.H.S. | en_US |
dc.subject.mesh | DNA, Viral/isolation & purification | en_US |
dc.subject.mesh | Electrophoresis, Capillary/methods | en_US |
dc.subject.mesh | Plasmids/isolation & purification | en_US |
dc.title | Prediction of selectivity for the separation of double-stranded DNA fragments in electrophoresis | en_US |
dc.type | Article | en_US |
dc.coverage.spacial | United States | en_US |
dc.description.version | peer reviewed | en_US |
dc.rights.holder | Copyright © 1997 by ISC Technical Publications | en_US |