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dc.contributorWichita State University. Department of Chemistryen_US
dc.contributor.authorWimalasena, Kandategeen_US
dc.contributor.authorWimalasena, D. Shyamalien_US
dc.date.accessioned2012-02-06T17:17:13Z
dc.date.available2012-02-06T17:17:13Z
dc.date.issued1991-03-29en_US
dc.identifier2025264en_US
dc.identifier0372516en_US
dc.identifier0006-291X(91)91653-Ten_US
dc.identifier.citationBiochemical and biophysical research communications. 1991 Mar 29; 175(3): 920-7.en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10057/4400
dc.descriptionFull text of this article is not available in SOAR.en_US
dc.description.abstractDopamine beta-monooxygenase is shown to catalyze the oxidation of N,N,N',N'-tetramethyl-1,4-phenylenediamine (TMPD) to its cation radical in the presence of a regular substrate and molecular oxygen. The enzyme-mediated oxidation of TMPD is stoichiometrically coupled with the hydoxylation of the substrate to the corresponding enzymatic product. TMPD is kinetically well behaved as an alternate electron donor for the enzyme with a potency comparable to that of the most efficient electron donor, ascorbate. Dopamine beta-monooxygenase mediated oxidation of TMPD has been employed to design a convenient and sensitive spectrophotometric assay for the enzyme. The finding that TMPD is a well behaved facile alternate electron donor for dopamine beta-monooxygenase raises some interesting novel questions regarding the specificity and chemistry of the reduction site, which may have important implications on the reduction of active site coppers of the enzyme.en_US
dc.format.extent920-7en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesBiochemical and biophysical research communicationsen_US
dc.relation.ispartofseriesBiochem. Biophys. Res. Commun.en_US
dc.sourceNLMen_US
dc.subjectComparative Studyen_US
dc.subjectResearch Support, Non-U.S. Gov'ten_US
dc.subject.meshDopamine beta-Hydroxylase/metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshOxygen Consumptionen_US
dc.subject.meshSpectrophotometry/methodsen_US
dc.subject.meshSubstrate Specificityen_US
dc.subject.meshTetramethylphenylenediamine/metabolismen_US
dc.subject.meshTyramine/metabolismen_US
dc.titleN,N,N',N'-tetramethyl-1,4-phenylenediamine: a facile electron donor and chromophoric substrate for dopamine beta-monooxygenaseen_US
dc.typeArticleen_US
dc.coverage.spacialUnited Statesen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright © 1991, Elsevieren_US


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