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dc.contributorWichita State University. Department of Chemistryen_US
dc.contributor.authorSinghal, Ram P.en_US
dc.contributor.authorOtim, Ochanen_US
dc.date.accessioned2012-02-06T17:17:08Z
dc.date.available2012-02-06T17:17:08Z
dc.date.issued2000-05-27en_US
dc.identifier10872835en_US
dc.identifier0372516en_US
dc.identifierS0006-291X(00)92720-7en_US
dc.identifier.citationBiochemical and biophysical research communications. 2000 May 27; 272(1): 251-8.en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://dx.doi.org/10.1006/bbrc.2000.2720en_US
dc.identifier.urihttp://hdl.handle.net/10057/4398
dc.descriptionClick on the DOI link below to access the article (may not be free).en_US
dc.description.abstractThis work deals with annealing of single-stranded DNA and the binding of a serum respond factor to a DNA probe containing specific binding site. Capillary electrophoresis (CE) method is explored and compared with the mobility-shift gel electrophoresis (GE) procedure. The results indicate the CE method offers direct and rapid annealing of the DNA strands. It requires no prior incubation with additives (polynucleotides, proteins) to reduce nonspecific DNA-protein interactions. Unwanted nonspecific interactions are not observed in the CE method. The presence of a fluorescein tag to the DNA probe yields identical results to those with the radioactive label. A fluorescein tag in the CE work can be used without any adverse effects. The dissociation constant (Kd) of this protein-DNA complex by the CE method was similar to those determined by the GE method (approximately 10(-6) M). The proposed method is extremely powerful, highly sensitive, quantitative, and fast. It can determine even very small conformational differences of the DNA probe.en_US
dc.format.extent251-8en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesBiochemical and biophysical research communicationsen_US
dc.relation.ispartofseriesBiochem. Biophys. Res. Commun.en_US
dc.sourceNLMen_US
dc.subjectResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshDNA/isolation & purificationen_US
dc.subject.meshDNA Probes/chemistryen_US
dc.subject.meshDNA, Single-Stranded/isolation & purificationen_US
dc.subject.meshDNA-Binding Proteinsen_US
dc.subject.meshElectrophoresis, Capillary/methodsen_US
dc.subject.meshFluoresceinsen_US
dc.subject.meshHumansen_US
dc.subject.meshMicellesen_US
dc.subject.meshNuclear Proteinsen_US
dc.subject.meshPolydeoxyribonucleotidesen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshSerum Response Factoren_US
dc.subject.meshDNA/metabolismen_US
dc.subject.meshDNA, Single-Stranded/metabolismen_US
dc.titleDNA annealing and DNA-protein interactions by capillary electrophoresisen_US
dc.typeArticleen_US
dc.coverage.spacialUnited Statesen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright © 2000, Elsevieren_US


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