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dc.contributorWichita State University. Department of Chemistryen_US
dc.contributor.authorSinghal, Ram P.en_US
dc.contributor.authorXian, Junen_US
dc.contributor.authorOtim, Ochanen_US
dc.identifier.citationJournal of chromatography. A. 1996 Dec 20; 756(1-2): 263-77.en_US
dc.descriptionFull text of this article is not available in SOAR.en_US
dc.description.abstractSoluble polymers of linear chains with limited branching and spherical polymers (limit dextrins and sucrose, such as Dextran and Ficoll (Pharmacia Chemicals), yielding lower viscosities, are examined here for the separation of different nucleotides and several anti-AIDS drugs by capillary zone electrophoresis (CZE). The linear polymer forms a network but spherical polymers appear to create a second pseudo-phase. In general, they tend to enhance the solute mobility and reduce peak width; thus, they improve the column efficiency. We observe that the beads of a spherical polymer produce a pseudo-phase even in a very low polymer concentration. The proposed method involving a spherical polymer yields the best separation for twelve deoxyribonucleoside mono-, di- and triphosphates in ca. 10 min. Common anti-AIDS drugs (ddA, ddC, ddI, d4T, AZT) and an AZT metabolite (AZT-glucuronate) are resolved by using conventional micellar electrokinetic capillary chromatography (MEKC). These results not only offer fast and highly sensitive detection techniques for the pharmacokinetics of nucleotides, drugs, and their metabolites, but they also demonstrate an application of the proposed second pseudo-phase involving spherical polymer beads in CZE separations.en_US
dc.relation.ispartofseriesJournal of chromatography. Aen_US
dc.relation.ispartofseriesJ Chromatogr Aen_US
dc.subjectResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshAnti-HIV Agents/isolation & purificationen_US
dc.subject.meshAntiviral Agents/isolation & purificationen_US
dc.subject.meshDeoxyribonucleotides/isolation & purificationen_US
dc.subject.meshElectrophoresis, Capillary/methodsen_US
dc.titleApplication of spherical and other polymers in capillary zone electrophoresis: separation of antiviral drugs and deoxyribonucleoside phosphates by different principlesen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright © 1996 by Elsevieren_US

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